TLN1 Knockout cell line(NB4)

TLN1 Knockout cell line(NB4) Order

Catalog#

YKO-H400

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

TLN1 Knockout cell line (NB4)

Morphology

Monocyte, suspension

Passage ratio

1：2-1：4

Species

Human

Gene Name

TLN1

Gene ID

7094

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%RPMI-1640+10% FBS

Target region

Knockout region: Exon 6 (based on transcript TLN1-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:NB4			STR Info (Cell bank) Cell Line:NB-4
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	13	13		13	13
D13S317	11	12		11	12
D7S820	10	13	14.0	10	13
D16S539	9	9		9	9
VWA	16	19		16	19
TH01	7	9.3		7	9.3
AMEL	X	X		X	X
TPOX	8	11		8	11
CSF1PO	11	12		11	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TLN1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the NB4 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TLN1 gene knockout TLN1 Knockout cell line (NB4) will be expanded and cryopreserved.

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